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RELB Polyclonal antibody

RELB Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 29601-1-AP

Synonyms

Transcription factor RelB, I-Rel

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RELB Polyclonal antibody (29601-1-AP)

    WB analysis using 29601-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29601-1-AP (RELB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Raji cells using RELB Polyclonal antibody (29601-1-AP)

    IP experiment of Raji using 29601-1-AP

    IP result of anti-RELB (IP:29601-1-AP, 4ug; Detection:29601-1-AP 1:1000) with Raji cells lysate 2840 ug.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using RELB Polyclonal antibody (29601-1-AP)

    IHC staining of human ovary cancer using 29601-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29601-1-AP (RELB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inRaji cells, Ramos cells
Positive IP detected inRaji cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29601-1-AP targets RELB in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RELB fusion protein Ag30334
Full Name v-rel reticuloendotheliosis viral oncogene homolog B
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 62-65 kDa
GenBank Accession NumberBC028013
Gene Symbol RELB
Gene ID (NCBI) 5971
RRIDAB_2918330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RELB is one of the more unusual members of the NF-κB family, it is best known for its roles in lymphoid development, DC biology, and noncanonical signaling. RELB is capable of direct binding to the AhR that supports the xenobiotic-detoxifying pathway. RELB can regulate the circadian rhythm by directly binding to the BMAL partner of CLOCK.

Protocols

Product Specific Protocols
WB protocol for RELB antibody 29601-1-APDownload protocol
IHC protocol for RELB antibody 29601-1-APDownload protocol
IP protocol for RELB antibody 29601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols