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CDCA5 Polyclonal antibody

CDCA5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29316-1-AP

Synonyms

Sororin, p35, Cell division cycle-associated protein 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDCA5 Polyclonal antibody (29316-1-AP)

    WB analysis using 29316-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29316-1-AP (CDCA5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CDCA5 Polyclonal antibody (29316-1-AP)

    IHC staining of human skin cancer using 29316-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29316-1-AP (CDCA5 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CDCA5 Polyclonal antibody (29316-1-AP)

    IHC staining of human skin cancer using 29316-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29316-1-AP (CDCA5 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDCA5 Polyclonal antibody (29316-1-AP)

    IF Staining of HeLa using 29316-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDCA5 antibody (29316-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, LNCaP cells, U2OS cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29316-1-AP targets CDCA5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDCA5 fusion protein Ag30966
Full Name cell division cycle associated 5
Calculated Molecular Weight 27 kDa
Observed Molecular Weight27~35 kDa
GenBank Accession NumberBC011000
Gene Symbol CDCA5
Gene ID (NCBI) 113130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human cell division cycle associated 5 (CDCA5), also known as Sororin, is required for stable binding of cohesin to chromatid in the S and G2/M phases and degraded through anaphase-promoting complex-dependent ubiquitination in the G0/G1 phase (PMID: 30808873). Suppression of CDCA5 expression with siRNAs inhibited the growth of lung cancer cells. CDCA5 positivity is an independent prognostic factor for lung cancer patients (PMID: 20551060). The predicted molecular size of CDCA is 27 kd, with an electrophoretic mobility of ~35 kd on SDS-PAGE, possibly due to its isoelectric point (pI) of approximately 10 (PMID: 22580470).

Protocols

Product Specific Protocols
WB protocol for CDCA5 antibody 29316-1-APDownload protocol
IHC protocol for CDCA5 antibody 29316-1-APDownload protocol
IF protocol for CDCA5 antibody 29316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols