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Phospho-STAT1 (Tyr701) Polyclonal antibody

Phospho-STAT1 (Tyr701) Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 28979-1-AP

Synonyms

STAT1, Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-STAT1 (Tyr701) Polyclonal antibody (28979-1-AP)

    WB analysis using 28979-1-AP

    Non-treated THP-1 cells, IFN gamma and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 28979-1-AP (Phospho-STAT1 (Tyr701) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-STAT1 (Tyr701) Polyclonal antibody (28979-1-AP)

    IF Staining of HeLa using 28979-1-AP

    Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using Phospho-STAT1 (Tyr701) antibody (28979-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of THP-1 cells using Phospho-STAT1 (Tyr701) Polyclonal antibody (28979-1-AP)

    FC experiment of THP-1 using 28979-1-AP

    1x10^6 IFN gamma treated THP-1 cells were intracellularly stained with 0.25 ug Phospho-STAT1 (Tyr701) Polyclonal antibody (28979-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Tested Applications

Positive WB detected inIFN gamma and LPS treated THP-1 cells
Positive IF/ICC detected inIFN alpha treated HeLa cells
Positive FC (Intra) detected inIFN gamma treated THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28979-1-AP targets Phospho-STAT1 (Tyr701) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name signal transducer and activator of transcription 1, 91kDa
Calculated Molecular Weight 83 kDa
Observed Molecular Weight84-91 kDa
GenBank Accession NumberBC002704
Gene Symbol STAT1
Gene ID (NCBI) 6772
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. The phosphorylation of STAT1 at Tyr701 promotes the dimerization of STAT1 and its translocation into the nucleus where it binds the GAS promoter to allow the transcription of inflammatory response genes(PMID: 31408005). The transcript of STAT1 undergoes alternative splicing, resulting in two isoforms: STAT1α (91 kDa) and STAT1β (84 kDa)(PMID: 20159032).

Protocols

Product Specific Protocols
WB protocol for Phospho-STAT1 (Tyr701) antibody 28979-1-APDownload protocol
IF protocol for Phospho-STAT1 (Tyr701) antibody 28979-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Transl Med

Pretreatment of UC-MSCs with IFN-α2 improves treatment of liver fibrosis by recruiting neutrophils

Authors - Ye Xie
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Agnieszka (Verified Customer) (02-28-2023)

great working antibody, gives the background

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000