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UCKL1 Polyclonal antibody

UCKL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28762-1-AP

Synonyms

UCK1 LIKE, UCK1L, UCKL1, Uridine cytidine kinase like 1, URKL1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UCKL1 Polyclonal antibody (28762-1-AP)

    WB analysis using 28762-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28762-1-AP (UCKL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UCKL1 Polyclonal antibody (28762-1-AP)

    IHC staining of human liver cancer using 28762-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28762-1-AP (UCKL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using UCKL1 Polyclonal antibody (28762-1-AP)

    IHC staining of human liver cancer using 28762-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28762-1-AP (UCKL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, Raji cells, K-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28762-1-AP targets UCKL1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UCKL1 fusion protein Ag30535
Full Name uridine-cytidine kinase 1-like 1
Calculated Molecular Weight 419 aa, 46 kDa
Observed Molecular Weight61 kDa
GenBank Accession NumberBC033078
Gene Symbol UCKL1
Gene ID (NCBI) 54963
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Uridine cytidine kinase like-1 (UCKL-1) is a largely uncharacterized protein over-expressed in many tumor cells, especially in highly malignant, aggressive tumors. Sequence analysis indicates that UCKL-1 has homology to uridine kinases, enzymes that play a role in DNA and RNA synthesis and that are often up-regulated in tumor cells. Previous studies have shown that UCKL-1 is a substrate for natural killer lytic-associated molecule (NKLAM), an E3 ubiquitin ligase found in NK cell cytolytic granules. Ubiquitination of UCKL-1 by NKLAM leads to its degradation. Increased expression of NKLAM enhances NK-mediated tumoricidal activity. (PMID: 32083188)

Protocols

Product Specific Protocols
WB protocol for UCKL1 antibody 28762-1-APDownload protocol
IHC protocol for UCKL1 antibody 28762-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols