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  • Featured Product
  • KD/KO Validated

SCD Polyclonal antibody

SCD Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28678-1-AP

Synonyms

Acyl CoA desaturase, Delta(9) desaturase, FADS5, Fatty acid desaturase, MSTP008, SCD, SCD1, SCDOS, Stearoyl CoA desaturase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SCD Polyclonal antibody (28678-1-AP)

    WB analysis using 28678-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28678-1-AP (SCD antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using SCD Polyclonal antibody (28678-1-AP)

    WB analysis of A431 using 28678-1-AP

    WB result of SCD antibody (28678-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCD transfected A431 cells.

  • Immunohistochemistry (IHC) staining of human liver tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of human liver using 28678-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of human liver using 28678-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of mouse liver using 28678-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of mouse liver using 28678-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of mouse brown adipose using 28678-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of mouse brown adipose using 28678-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of human colon cancer using 28678-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of human ovary tumor using 28678-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of human stomach cancer using 28678-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using SCD Polyclonal antibody (28678-1-AP)

    IHC staining of rat heart using 28678-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 28678-1-AP (SCD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SCD Polyclonal antibody (28678-1-AP)

    IF Staining of MCF-7 using 28678-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SCD antibody (28678-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA431 cells, A375 cells, A549 cells, HepG2 cells
Positive IHC detected inmouse liver tissue, human liver tissue, mouse brown adipose tissue, human colon cancer tissue, human ovary tumor tissue, human stomach cancer tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28678-1-AP targets SCD in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCD fusion protein Ag29156
Full Name stearoyl-CoA desaturase (delta-9-desaturase)
Calculated Molecular Weight 355 aa, 41 kDa
Observed Molecular Weight28-42 kDa
GenBank Accession NumberBC005807
Gene Symbol SCD
Gene ID (NCBI) 6319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SCD1 (stearoyl-CoA desaturase) is a microsomal fatty acid monodesaturase, which catalyses the committed step in the biosynthesis of mono-unsaturated fatty acids from saturated fatty acids (PMID:10946019). SCD1 and SCD2 are the main isoforms expressed in mouse liver and brain respectively (PMID:15907797). The formation of homodimers and oligomers is an intrinsic property of SCD proteins. SCD1 is a multi-pass membrane protein and detected double bands of 37-42 kDa. The degradation product of 28 kDa may be caused by a major cleavage site at the C-terminus (PMID:15610069, PMID: 9843580).

Protocols

Product Specific Protocols
WB protocol for SCD antibody 28678-1-APDownload protocol
IHC protocol for SCD antibody 28678-1-APDownload protocol
IF protocol for SCD antibody 28678-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

7-Dehydrocholesterol dictates ferroptosis sensitivity

Authors - Yaxu Li
View Article
human,mouseWB,IHC

Adv Sci (Weinh)

Transcriptional Regulation of De Novo Lipogenesis by SIX1 in Liver Cancer Cells

Authors - Ling Li
View Article
humanWB

Free Radic Biol Med

Zinc deficiency drives ferroptosis resistance by lactate production in esophageal squamous cell carcinoma

Authors - Peiyan Yang
View Article
mouseWB

Sci Rep

MARK4 aggravates cardiac dysfunction in mice with STZ-induced diabetic cardiomyopathy by regulating ACSL4-mediated myocardial lipid metabolism

Authors - Yi Wu
View Article
humanWB

Carcinogenesis

Macropinocytosis inhibits alkaliptosis in pancreatic cancer cells through fatty acid uptake

Authors - Fangquan Chen
View Article
mouseWB

Sci Adv

Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis

Authors - Shuwei Hu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Marco (Verified Customer) (07-09-2024)

It does its job in NRVCMs

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: NRVCMs