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PRKAR2B Polyclonal antibody

PRKAR2B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 28351-1-AP

Synonyms

RII BETA, PRKAR2, cAMP-dependent protein kinase type II-beta regulatory subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRKAR2B Polyclonal antibody (28351-1-AP)

    WB analysis using 28351-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28351-1-AP (PRKAR2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PRKAR2B Polyclonal antibody (28351-1-AP)

    WB analysis using 28351-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28351-1-AP (PRKAR2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PRKAR2B Polyclonal antibody (28351-1-AP)

    IP experiment of mouse brain using 28351-1-AP

    IP result of anti-PRKAR2B (IP:28351-1-AP, 4ug; Detection:28351-1-AP 1:1000) with mouse brain tissue lysate 1080 ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PRKAR2B Polyclonal antibody (28351-1-AP)

    IHC staining of human stomach cancer using 28351-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28351-1-AP (PRKAR2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using PRKAR2B Polyclonal antibody (28351-1-AP)

    IHC staining of human stomach using 28351-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28351-1-AP (PRKAR2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using PRKAR2B Polyclonal antibody (28351-1-AP)

    IF Staining of Neuro-2a using 28351-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using PRKAR2B Polyclonal antibody (28351-1-AP)

    IF Staining of Neuro-2a using 28351-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using PRKAR2B Polyclonal antibody (28351-1-AP)

    IF Staining of SH-SY5Y using 28351-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using PRKAR2B Polyclonal antibody (28351-1-AP)

    FC experiment of SH-SY5Y using 28351-1-AP

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human PRKAR2B (28351-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inY79 cells, human testis tissue, mouse brain tissue, mouse eye tissue, rat brain tissue, mouse testis tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNeuro-2a cells, SH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28351-1-AP targets PRKAR2B in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKAR2B fusion protein Ag28787
Full Name protein kinase, cAMP-dependent, regulatory, type II, beta
Calculated Molecular Weight 418 aa, 46 kDa
Observed Molecular Weight46-50 kDa
GenBank Accession NumberBC075800
Gene Symbol PRKAR2B
Gene ID (NCBI) 5577
RRIDAB_2918156
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PRKAR2B antibody 28351-1-APDownload protocol
IHC protocol for PRKAR2B antibody 28351-1-APDownload protocol
IF protocol for PRKAR2B antibody 28351-1-APDownload protocol
IP protocol for PRKAR2B antibody 28351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Inflamm Res

Comprehensive Analysis of Fatty Acid Metabolism in Diabetic Nephropathy from the Perspective of Immune Landscapes, Diagnosis and Precise Therapy

Authors - Enyi Zhu
View Article