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PTPRK Polyclonal antibody

PTPRK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28151-1-AP

Synonyms

DKFZp686C2268, DKFZp779N1045, PTPK, PTPRK, R PTP kappa

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPRK Polyclonal antibody (28151-1-AP)

    WB analysis using 28151-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28151-1-AP (PTPRK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PTPRK Polyclonal antibody (28151-1-AP)

    WB analysis using 28151-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 28151-1-AP (PTPRK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse spleen tissue, rat spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28151-1-AP targets PTPRK in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPRK fusion protein Ag27765
Full Name protein tyrosine phosphatase, receptor type, K
Calculated Molecular Weight 1439 aa, 162 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC140775
Gene Symbol PTPRK
Gene ID (NCBI) 5796
RRIDAB_2881074
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPRK (Protein Tyrosine Phosphatase Receptor Type Kappa), a member of the receptor-type protein tyrosine phosphatase family, is a transmembrane protein that regulates cell-cell contact. PTPRK is a 210 kDa precursor protein and converted by furin to a mature heterodimeric protein composed of a non-covalently attached amino-terminal extracellular subunit (E-subunit, 120 kDa) and carboxyl-terminal transmembrane subunit (P-subunit, 95 kDa) (PMID: 24882578, 16648485). Loss of PTPRK activity has been observed in pancreatic cancer, primary CNS lymphoma and melanoma, and is associated with poor survival of cancer patients, which suggest that PTPRK is a potential tumor suppressor (PMID: 23696788).

Protocols

Product Specific Protocols
WB protocol for PTPRK antibody 28151-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols