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KLK15 Polyclonal antibody

KLK15 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27515-1-AP

Synonyms

ACO, ACO protease, HSRNASPH, Kallikrein 15, KLK15

Validation Data Gallery

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  • Western Blot (WB) analysis of SMMC-7721 cells using KLK15 Polyclonal antibody (27515-1-AP)

    WB analysis of SMMC-7721 using 27515-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 27515-1-AP (KLK15 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using KLK15 Polyclonal antibody (27515-1-AP)

    IHC staining of human liver cancer using 27515-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27515-1-AP (KLK15 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using KLK15 Polyclonal antibody (27515-1-AP)

    IHC staining of human liver cancer using 27515-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27515-1-AP (KLK15 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using KLK15 Polyclonal antibody (27515-1-AP)

    IHC staining of human liver using 27515-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27515-1-AP (KLK15 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using KLK15 Polyclonal antibody (27515-1-AP)

    IHC staining of human liver using 27515-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27515-1-AP (KLK15 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inSMMC-7721 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27515-1-AP targets KLK15 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLK15 fusion protein Ag26232
Full Name kallikrein-related peptidase 15
Calculated Molecular Weight 255 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC069480
Gene Symbol KLK15
Gene ID (NCBI) 55554
RRIDAB_2880895
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for KLK15 antibody 27515-1-APDownload protocol
IHC protocol for KLK15 antibody 27515-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols