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RHOA Polyclonal antibody

RHOA Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27213-1-AP

Synonyms

ARHA, ARH12, RHO12, Rho cDNA clone 12, h12

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse kidney tissue using RHOA Polyclonal antibody (27213-1-AP)

    WB analysis of mouse kidney using 27213-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27213-1-AP (RHOA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using RHOA Polyclonal antibody (27213-1-AP)

    IHC staining of human stomach using 27213-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27213-1-AP (RHOA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse kidney tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27213-1-AP targets RHOA in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHOA fusion protein Ag25873
Full Name ras homolog gene family, member A
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC005976
Gene Symbol RHOA
Gene ID (NCBI) 387
RRIDAB_2880803
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.

Protocols

Product Specific Protocols
WB protocol for RHOA antibody 27213-1-APDownload protocol
IHC protocol for RHOA antibody 27213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Med Rep

Protective effects of curcumin against rat intestinal inflammation‑related motility disorders.

Authors - Yang Yao
View Article