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DHRS7C Polyclonal antibody

DHRS7C Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26020-1-AP

Synonyms

DHRS7C, SDR32C2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DHRS7C Polyclonal antibody (26020-1-AP)

    WB analysis using 26020-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26020-1-AP (DHRS7C antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using DHRS7C Polyclonal antibody (26020-1-AP)

    IHC staining of mouse skeletal muscle using 26020-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26020-1-AP (DHRS7C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using DHRS7C Polyclonal antibody (26020-1-AP)

    IHC staining of mouse heart using 26020-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26020-1-AP (DHRS7C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inrat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26020-1-AP targets DHRS7C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DHRS7C fusion protein Ag23392
Full Name dehydrogenase/reductase (SDR family) member 7C
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC147025
Gene Symbol DHRS7C
Gene ID (NCBI) 201140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DHRS7C antibody 26020-1-APDownload protocol
IHC protocol for DHRS7C antibody 26020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols