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RPS6KL1 Polyclonal antibody

RPS6KL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, hamster

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24757-1-AP

Synonyms

RPS6KL1

Validation Data Gallery

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  • Western Blot (WB) analysis of CHO cells using RPS6KL1 Polyclonal antibody (24757-1-AP)

    WB analysis of CHO using 24757-1-AP

    CHO cells were subjected to SDS PAGE followed by western blot with 24757-1-AP (RPS6KL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using RPS6KL1 Polyclonal antibody (24757-1-AP)

    IHC staining of human testis using 24757-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24757-1-AP (RPS6KL1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RPS6KL1 Polyclonal antibody (24757-1-AP)

    IHC staining of human testis using 24757-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24757-1-AP (RPS6KL1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using RPS6KL1 Polyclonal antibody (24757-1-AP)

    IHC staining of human colon using 24757-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24757-1-AP (RPS6KL1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using RPS6KL1 Polyclonal antibody (24757-1-AP)

    IHC staining of human colon using 24757-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24757-1-AP (RPS6KL1 Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inCHO cells
Positive IHC detected inhuman testis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24757-1-AP is RPS6KL1 Fusion Protein expressed in E. coli.

Tested Reactivity human, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS6KL1 fusion protein Ag15099
Full Name ribosomal protein S6 kinase-like 1
Calculated Molecular Weight 549 aa, 60 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC004540
Gene Symbol RPS6KL1
Gene ID (NCBI) 83694
RRIDAB_2879706
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPS6KL1 antibody 24757-1-APDownload protocol
IHC protocol for RPS6KL1 antibody 24757-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols