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IGFALS Polyclonal antibody

IGFALS Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 24545-1-AP

Synonyms

ALS, IGFALS

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using IGFALS Polyclonal antibody (24545-1-AP)

    WB analysis of human plasma using 24545-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24545-1-AP (IGFALS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman plasma

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24545-1-AP targets IGFALS in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IGFALS fusion protein Ag21696
Full Name insulin-like growth factor binding protein, acid labile subunit
Calculated Molecular Weight 605 aa, 66 kDa
Observed Molecular Weight85 kDa
GenBank Accession NumberBC025681
Gene Symbol IGFALS
Gene ID (NCBI) 3483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THE ACID-LABILE SUBUNIT (ALS), a liver-derived, GH-dependent, 85-kD glycoprotein, is a key component of the 150-kD IGF circulating ternary complex(PMID: 17726072). The protein encoded by this gene is a serum protein that binds insulin-like growth factors, increasing their half-life and their vascular localization. Production of the encoded protein, which contains twenty leucine-rich repeats, is stimulated by growth hormone. Defects in this gene are a cause of acid-labile subunit deficiency, which maifests itself in a delayed and slow puberty.

Protocols

Product Specific Protocols
WB protocol for IGFALS antibody 24545-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols