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WB analysis of mouse testis using 23372-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 23372-1-AP (UBQLN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 23372-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 23372-1-AP (UBQLN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 23372-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 23372-1-AP (UBQLN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 23372-1-AP
Immunohistochemical analysis of paraffin-embedded human testis slide using 23372-1-AP (UBQLN3 antibody at dilution of 1:50.
IHC staining of human testis using 23372-1-AP
Immunohistochemical analysis of paraffin-embedded human testis slide using 23372-1-AP (UBQLN3 antibody at dilution of 1:50.
IF Staining of HepG2 using 23372-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UBQLN3 antibody (23372-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

UBQLN3 Polyclonal antibody

Name: UBQLN3 Polyclonal antibody
Brand: Proteintech
SKU: 23372-1-AP

UBQLN3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 23372-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

UBQLN 3, Ubiquilin-3, ubiquilin3, ubiquilin 3, TUP1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse testis tissue, HepG2 cells, human testis tissue
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23372-1-AP targets UBQLN3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19982 Product name: Recombinant human Ubiquilin 3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 306-620 aa of BC036743 Sequence: WTSTHGGSGSRQGRQDGDQDAPDIRNRFPNFLGIIRLYDYLQQLHENPQSLGTYLQGTASALSQSQEPPPSVNRVPPSSPSSQEPGSGQPLPEESVAIKGRSSCPAFLRYPTENSTGQGGDQDGAGKSSTGHSTNLPDLVSGLGDSANRVPFAPLSFSPTAAIPGIPEPPWLPSPAYPRSLRPDGMNPAPQLQDEIQPQLPLLMHLQAAMANPRALQALRQIEQGLQVLATEAPRLLLWFMPCLAGTGSVAGGIESREDPLMSEDPLPNPPPEVFPALDSAELGFLSPPFLHMLQDLVSTNPQQLQPEAHFQVQL Predict reactive species
Full Name	ubiquilin 3
Calculated Molecular Weight	655 aa, 71 kDa
Observed Molecular Weight	71 kDa
GenBank Accession Number	BC036743
Gene Symbol	UBQLN3
Gene ID (NCBI)	50613
RRID	AB_2879268
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H347
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBQLN3(Ubiquilin-3) may interact with the testis-specific cyclin A1 and regulate cell cycle progression during spermatogenesis, as does frog Xdrp1, with which UBQLN3 shares sequence similarity(PMID:10831842).UBQLN3 expression is not observed in mouse eye, thyroid, pancreas,smooth muscle, ovary, submaxillary gland, uterus, and epididymis by northern blot(PMID:10831842 ).

Protocols

Product Specific Protocols
IF protocol for UBQLN3 antibody 23372-1-AP	Download protocol
IHC protocol for UBQLN3 antibody 23372-1-AP	Download protocol
WB protocol for UBQLN3 antibody 23372-1-AP	Download protocol