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GABARAP Polyclonal antibody

GABARAP Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22439-1-AP

Synonyms

GABARAP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GABARAP Polyclonal antibody (22439-1-AP)

    WB analysis using 22439-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22439-1-AP (GABARAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GABARAP Polyclonal antibody (22439-1-AP)

    WB analysis of mouse brain using 22439-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22439-1-AP (GABARAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using GABARAP Polyclonal antibody (22439-1-AP)

    WB analysis of SH-SY5Y using 22439-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22439-1-AP (GABARAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using GABARAP Polyclonal antibody (22439-1-AP)

    WB analysis of HEK-293 using 22439-1-AP

    Chloroquine treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 22439-1-AP (GABARAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, SH-SY5Y cells, Chloroquine treated HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22439-1-AP targets GABARAP in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GABARAP fusion protein Ag18209
Full Name GABA(A) receptor-associated protein
Calculated Molecular Weight 117 aa, 14 kDa
Observed Molecular Weight 19-21 kDa
GenBank Accession NumberBC106749
Gene Symbol GABARAP
Gene ID (NCBI) 11337
RRIDAB_2879106
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GABARAP antibody 22439-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other