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ARF3 Polyclonal antibody

ARF3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22289-1-AP

Synonyms

ADP-ribosylation factor 3, ADP ribosylation factor 3

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ARF3 Polyclonal antibody (22289-1-AP)

    WB analysis of mouse brain using 22289-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22289-1-AP (ARF3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using ARF3 Polyclonal antibody (22289-1-AP)

    WB analysis of rat brain using 22289-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 22289-1-AP (ARF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARF3 Polyclonal antibody (22289-1-AP)

    IHC staining of human breast cancer using 22289-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22289-1-AP (ARF3 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using ARF3 Polyclonal antibody (22289-1-AP)

    IHC staining of human breast cancer using 22289-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22289-1-AP (ARF3 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARF3 Polyclonal antibody (22289-1-AP)

    IF Staining of HeLa using 22289-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ARF3 antibody (22289-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22289-1-AP targets ARF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ADP-ribosylation factor 3
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberNM_001659
Gene Symbol ARF3
Gene ID (NCBI) 377
RRIDAB_2879061
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF3 is 95% homolouous to ARF1. Its function was not fully understood. (PMID: 7759471, PMID: 16042562).The antibody is specific to ARF3. If has no cross reaction to other ARF members.

Protocols

Product Specific Protocols
WB protocol for ARF3 antibody 22289-1-APDownload protocol
IHC protocol for ARF3 antibody 22289-1-APDownload protocol
IF protocol for ARF3 antibody 22289-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols