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B9D2 Polyclonal antibody

B9D2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22058-1-AP

Synonyms

B9 domain containing protein 2, B9 protein domain 2, B9D2, ICIS 1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human colon cancer tissue using B9D2 Polyclonal antibody (22058-1-AP)

    IHC staining of human colon cancer using 22058-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22058-1-AP (B9D2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using B9D2 Polyclonal antibody (22058-1-AP)

    IHC staining of human colon cancer using 22058-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22058-1-AP (B9D2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 22058-1-AP is B9D2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen B9D2 fusion protein Ag17104
Full Name B9 protein domain 2
Calculated Molecular Weight 175 aa, 19 kDa
GenBank Accession NumberBC004444
Gene Symbol B9D2
Gene ID (NCBI) 80776
RRIDAB_2878981
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for B9D2 antibody 22058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,zebrafishWB

Dis Model Mech

Variable phenotypes and penetrance between and within different zebrafish ciliary transition zone mutants

Authors - Jun Wang
View Article