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  • Featured Product
  • KD/KO Validated

SHP2 Polyclonal antibody

SHP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, Cell treatment

Conjugate

Unconjugated

Cat no : 20145-1-AP

Synonyms

PTPN11/SHP2, PTPN11, NS1, NS 1, EC:3.1.3.48

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPN11/SHP2 Polyclonal antibody (20145-1-AP)

    WB analysis using 20145-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20145-1-AP (PTPN11/SHP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PTPN11/SHP2 Polyclonal antibody (20145-1-AP)

    WB analysis using 20145-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20145-1-AP (SHP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PTPN11/SHP2 Polyclonal antibody (20145-1-AP)

    IP experiment of HEK-293 using 20145-1-AP

    IP result of anti-PTPN11/SHP2 (IP:20145-1-AP, 4ug; Detection:20145-1-AP 1:10000) with HEK-293 cells lysate 1575 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PTPN11/SHP2 Polyclonal antibody (20145-1-AP)

    IHC staining of human breast cancer using 20145-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20145-1-AP (SHP2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PTPN11/SHP2 Polyclonal antibody (20145-1-AP)

    IHC staining of human breast cancer using 20145-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20145-1-AP (SHP2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PTPN11/SHP2 Polyclonal antibody (20145-1-AP)

    IHC staining of human gliomas using 20145-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20145-1-AP (SHP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PTPN11/SHP2 Polyclonal antibody (20145-1-AP)

    IHC staining of human gliomas using 20145-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20145-1-AP (SHP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using PTPN11/SHP2 Polyclonal antibody (20145-1-AP)

    IHC staining of human heart using 20145-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20145-1-AP (SHP2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PTPN11/SHP2 Polyclonal antibody (20145-1-AP)

    IHC staining of human heart using 20145-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 20145-1-AP (SHP2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SHP2 Polyclonal antibody (20145-1-AP)

    IF Staining of HeLa using 20145-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SHP2 antibody (20145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, A431 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human gliomas tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20145-1-AP targets SHP2 in WB, IHC, IF/ICC, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHP2 fusion protein Ag13660
Full Name protein tyrosine phosphatase, non-receptor type 11
Calculated Molecular Weight 597 aa, 68 kDa
Observed Molecular Weight 68-75 kDa
GenBank Accession NumberBC008692
Gene Symbol PTPN11
Gene ID (NCBI) 5781
RRIDAB_10699877
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN11(protein tyrosine phosphatase, non-receptor type 11) is also named as PTP-1D, PTP2, PTP2C, PTP3, SHP2, CFC, CFC, BPTP3, SH-PTP2, SH-PTP3, MGC14433 and belongs to the protein-tyrosine phosphatase family and non-receptor class 2 subfamily. It modulates and regulates signaling through numerous pathways, many of which are active in the developing endocardial cushions and implicated the ERK pathway as a central mechanism(PMID:19017799). Its signaling may play equally important roles in retinal survival in both physiological and pathological conditions(PMID:21576358). Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1), Noonan syndrome type 1 (NS1), juvenile myelomonocytic leukemia (JMML) and metachondromatosis (MC). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for SHP2 antibody 20145-1-APDownload protocol
IHC protocol for SHP2 antibody 20145-1-APDownload protocol
IF protocol for SHP2 antibody 20145-1-APDownload protocol
IP protocol for SHP2 antibody 20145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Targeting NRAS via miR-1304-5p or farnesyltransferase inhibition confers sensitivity to ALK inhibitors in ALK-mutant neuroblastoma

Authors - Perla Pucci
View Article
ratWB

Hepatology

Shp2 promotes liver cancer stem cell expansion by augmenting β-catenin signaling and predicts chemotherapeutic response of patients.

Authors - Daimin Xiang
  • KD Validated
View Article
mouseWB

Oncogene

Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.

Authors - Y Patel
View Article
mouseCell treatment

Cell Chem Biol

Cellular signals converge at the NOX2-SHP-2 axis to induce reductive carboxylation in cancer cells.

Authors - Rukang Zhang
View Article
humanWB

Mol Ther Nucleic Acids

miR-552 Regulates Liver Tumor-Initiating Cell Expansion and Sorafenib Resistance.

Authors - Tao Han
View Article
humanWB

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
View Article