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TP53BP1-Specific Polyclonal antibody

TP53BP1-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20002-1-AP

Synonyms

TP53BP1, p53BP1, p53-binding protein 1, p53 binding protein 1, p202

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TP53BP1-Specific Polyclonal antibody (20002-1-AP)

    WB analysis using 20002-1-AP

    HeLa lysates were subjected to SDS PAGE followed by western blot with 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TP53BP1-Specific Polyclonal antibody (20002-1-AP)

    IHC staining of human stomach cancer using 20002-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using TP53BP1-Specific Polyclonal antibody (20002-1-AP)

    IHC staining of human stomach cancer using 20002-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 20002-1-AP (TP53BP1-Specific antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20002-1-AP targets TP53BP1-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name tumor protein p53 binding protein 1
Calculated Molecular Weight 214 kDa
Observed Molecular Weight450 kDa
GenBank Accession NumberNM_005657
Gene Symbol TP53BP1
Gene ID (NCBI) 7158
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TP53BP1, also named as p53BP1, p202, 53BP1 and FLJ41424, may have a role in checkpoint signaling during mitosis. TP53BP1 enhances TP53-mediated transcriptional activation. TP53BP1 plays a role in the response to DNA damage. This antibody is specific to TP53BP1.

Protocols

Product Specific Protocols
WB protocol for TP53BP1-Specific antibody 20002-1-APDownload protocol
IHC protocol for TP53BP1-Specific antibody 20002-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Heliyon

Cyclin-dependent kinase inhibitor 1A inhibits pyroptosis to enhance human lung adenocarcinoma cell radioresistance by promoting DNA repair

Authors - Jing Li
View Article