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KCNE4 Polyclonal antibody

KCNE4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18289-1-AP

Synonyms

KCNE4, MinK related peptide 3, MIRP3

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using KCNE4 Polyclonal antibody (18289-1-AP)

    WB analysis of L02 using 18289-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 18289-1-AP (KCNE4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

Tested Applications

Positive WB detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18289-1-AP targets KCNE4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name potassium voltage-gated channel, Isk-related family, member 4
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberNM_080671
Gene Symbol KCNE4
Gene ID (NCBI) 23704
RRIDAB_2132300
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCNE4, also named as MIRP3 and MGC2035, is a membrane protein belongs to a family of single transmembrane domain proteins known to have dramatic effect on the gating of certain potassium channels. KCNE4 is expressed strongly in heart, skeletal muscle and kidney. Electrophysiological studies show that human KCNE4 modulates the activation of the KCNQ1 channel.

Protocols

Product Specific Protocols
WB protocol for KCNE4 antibody 18289-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Cell Sci

The C-terminal domain of Kv1.3 regulates functional interactions with the KCNE4 subunit.

Authors - Laura Solé
View Article
humanWB

Sci Rep

Calmodulin-dependent KCNE4 dimerization controls membrane targeting.

Authors - Sara R Roig
View Article
humanIHC

Sci Rep

KCNE4-dependent functional consequences of Kv1.3-related leukocyte physiology.

Authors - Albert Vallejo-Gracia
View Article
humanWB,IF

FASEB J

The calmodulin-binding tetraleucine motif of KCNE4 is responsible for association with Kv1.3.

Authors - Laura Solé
View Article