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DDX19B Polyclonal antibody

DDX19B Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 18285-1-AP

Synonyms

DBP5, DDX19, DDX19B, DEAD box protein 19B, DEAD box RNA helicase DEAD5, RNAh

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using DDX19B Polyclonal antibody (18285-1-AP)

    WB analysis of K-562 using 18285-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using DDX19B Polyclonal antibody (18285-1-AP)

    WB analysis of HEK-293 using 18285-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using DDX19B Polyclonal antibody (18285-1-AP)

    WB analysis of NIH/3T3 using 18285-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DDX19B Polyclonal antibody (18285-1-AP)

    WB analysis of HeLa using 18285-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18285-1-AP (DDX19B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using DDX19B Polyclonal antibody (18285-1-AP)

    IP experiment of K-562 using 18285-1-AP

    IP result of anti-DDX19B (IP:18285-1-AP, 4ug; Detection:18285-1-AP 1:500) with K-562 cells lysate 4000ug.

Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, HeLa cells, NIH/3T3 cells
Positive IP detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18285-1-AP targets DDX19B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDX19B fusion protein Ag13165
Full Name DEAD (Asp-Glu-Ala-As) box polypeptide 19B
Calculated Molecular Weight479aa,54 kDa; 370aa,42 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC010008
Gene Symbol DDX19B
Gene ID (NCBI) 11269
RRIDAB_10597088
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DDX19B antibody 18285-1-APDownload protocol
IP protocol for DDX19B antibody 18285-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Acta Neuropathol Commun

Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease

Authors - Suman Rimal
View Article