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CFP Polyclonal antibody

CFP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17192-1-AP

Synonyms

BFD, CFP, Complement factor P, complement factor properdin, PFC, PFD, PROPERDIN

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CFP Polyclonal antibody (17192-1-AP)

    WB analysis of Jurkat using 17192-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17192-1-AP (CFP Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human spleen tissue using CFP Polyclonal antibody (17192-1-AP)

    IHC staining of human spleen using 17192-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17192-1-AP (CFP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CFP Polyclonal antibody (17192-1-AP)

    IHC staining of human spleen using 17192-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17192-1-AP (CFP Antibody) at dilution of 1:50 (under 10x lens).

Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17192-1-AP is CFP Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CFP fusion protein Ag9798
Full Name complement factor properdin
Calculated Molecular Weight 469 aa, 51 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC015756
Gene Symbol CFP
Gene ID (NCBI) 5199
RRIDAB_2878359
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CFP, also named as Properdin or CFP, is a 469 amino acid protein, which contains 7 TSP type-1 domains. CFP as secreted protein is a positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Properdin deficiency poses a significant risk for severe meningococcal infections (PMID: 22229731). Properdin may be novel biomarker for future risk of type 2 diabetes (PMID: 22338105).

Protocols

Product Specific Protocols
WB protocol for CFP antibody 17192-1-APDownload protocol
IHC protocol for CFP antibody 17192-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.

Authors - Anika Alberts
View Article
humanIHC

J Cancer

CFP is a prognostic biomarker and correlated with immune infiltrates in Gastric Cancer and Lung Cancer.

Authors - Guoliang Cui
View Article
humanWB

Cell Death Dis

Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration

Authors - Yunfeng Hu
View Article