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MYL6B Polyclonal antibody

MYL6B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16963-1-AP

Synonyms

MLC1SA, MYL6B, Myosin light chain 6B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MYL6B Polyclonal antibody (16963-1-AP)

    WB analysis using 16963-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16963-1-AP (MYL6B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYL6B Polyclonal antibody (16963-1-AP)

    IHC staining of mouse skeletal muscle using 16963-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16963-1-AP (MYL6B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using MYL6B Polyclonal antibody (16963-1-AP)

    IHC staining of mouse skeletal muscle using 16963-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 16963-1-AP (MYL6B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHuH-7 cells, mouse testis tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16963-1-AP targets MYL6B in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYL6B fusion protein Ag10683
Full Name myosin, light chain 6B, alkali, smooth muscle and non-muscle
Calculated Molecular Weight 208 aa, 23 kDa
Observed Molecular Weight 22-25 kDa
GenBank Accession NumberBC012425
Gene Symbol MYL6B
Gene ID (NCBI) 140465
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYL6B, also known as MLC1SA, is primarily found in a hexamer consisting of four light chains and two heavy chains. MYL6B is an essential light chain for non-muscle Myosin II (NMII) that is involved in the control of cell adhesion, cell migration and tissue architecture, cargo transport, and endocytosis (PMID: 29439719, 33817240).

Protocols

Product Specific Protocols
WB protocol for MYL6B antibody 16963-1-APDownload protocol
IHC protocol for MYL6B antibody 16963-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols