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MRPS28 Polyclonal antibody

MRPS28 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16378-1-AP

Synonyms

MRPS35, MRP-S28, MRPS 28, MRP S35, MRP S28

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MRPS28 Polyclonal antibody (16378-1-AP)

    WB analysis using 16378-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 16378-1-AP (MRPS28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using MRPS28 Polyclonal antibody (16378-1-AP)

    IHC staining of human liver using 16378-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16378-1-AP (MRPS28 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using MRPS28 Polyclonal antibody (16378-1-AP)

    IHC staining of human liver using 16378-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16378-1-AP (MRPS28 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MRPS28 Polyclonal antibody (16378-1-AP)

    IF Staining of HepG2 using 16378-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16378-1-AP (MRPS28 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MRPS28 Polyclonal antibody (16378-1-AP)

    IF Staining of HeLa using 16378-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16378-1-AP (MRPS28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16378-1-AP targets MRPS28 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MRPS28 fusion protein Ag9354
Full Name mitochondrial ribosomal protein S28
Calculated Molecular Weight 187 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC010150
Gene Symbol MRPS28
Gene ID (NCBI) 28957
RRIDAB_2180748
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MRPS28 antibody 16378-1-APDownload protocol
IHC protocol for MRPS28 antibody 16378-1-APDownload protocol
IF protocol for MRPS28 antibody 16378-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Mutations in the MRPS28 gene encoding the small mitoribosomal subunit protein bS1m in a patient with intrauterine growth retardation, craniofacial dysmorphism and multisystemic involvement.

Authors - Juliette Pulman
View Article