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PLA2G1B Polyclonal antibody

PLA2G1B Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 15843-1-AP

Synonyms

PLA2, Phosphatidylcholine 2-acylhydrolase 1B, EC:3.1.1.4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PLA2G1B Polyclonal antibody (15843-1-AP)

    WB analysis using 15843-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15843-1-AP (PLA2G1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using PLA2G1B Polyclonal antibody (15843-1-AP)

    WB analysis of mouse spleen using 15843-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15843-1-AP (PLA2G1B Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PLA2G1B Polyclonal antibody (15843-1-AP)

    IHC staining of human pancreas cancer using 15843-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15843-1-AP (PLA2G1B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PLA2G1B Polyclonal antibody (15843-1-AP)

    IHC staining of human pancreas cancer using 15843-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15843-1-AP (PLA2G1B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using PLA2G1B Polyclonal antibody (15843-1-AP)

    IF Staining of mouse pancreas using 15843-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using PLA2G1B antibody (15843-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse pancreas tissue, mouse spleen tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15843-1-AP targets PLA2G1B in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PLA2G1B fusion protein Ag8603
Full Name phospholipase A2, group IB (pancreas)
Calculated Molecular Weight70aa,8 kDa; 148aa,16 kDa
Observed Molecular Weight 13-16 kDa
GenBank Accession NumberBC005386
Gene Symbol PLA2G1B
Gene ID (NCBI) 5319
RRIDAB_2878190
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLA2G1B is a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. PLA2G1B catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids.

Protocols

Product Specific Protocols
WB protocol for PLA2G1B antibody 15843-1-APDownload protocol
IHC protocol for PLA2G1B antibody 15843-1-APDownload protocol
IF protocol for PLA2G1B antibody 15843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols