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CHPF-N terminal Polyclonal antibody

CHPF-N terminal Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15801-1-AP

Synonyms

ChPF, Chondroitin-polymerizing factor, EC:2.4.1.175, CSS2, CHSY2

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using CHPF-N terminal Polyclonal antibody (15801-1-AP)

    WB analysis of human brain using 15801-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15801-1-AP (CHPF-N terminal antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CHPF-N terminal Polyclonal antibody (15801-1-AP)

    WB analysis of human brain using 15801-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15801-1-AP (CHPF-N terminal antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CHPF-N terminal Polyclonal antibody (15801-1-AP)

    IHC staining of human skin cancer using 15801-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15801-1-AP (CHPF-N terminal antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15801-1-AP targets CHPF-N terminal in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHPF-N terminal fusion protein Ag8523
Full Name chondroitin polymerizing factor
Calculated Molecular Weight 775 aa, 85 kDa
Observed Molecular Weight 85-90 kDa, 65-70 kDa
GenBank Accession NumberBC008878
Gene Symbol CHPF
Gene ID (NCBI) 79586
RRIDAB_2291727
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CHPF-N terminal antibody 15801-1-APDownload protocol
IHC protocol for CHPF-N terminal antibody 15801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols