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NDUFC2 Polyclonal antibody

NDUFC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15573-1-AP

Synonyms

HLC 1, Complex I-B14.5b, Complex I B14.5b, CI-B14.5b, CI B14.5b

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NDUFC2 Polyclonal antibody (15573-1-AP)

    WB analysis using 15573-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NDUFC2 Polyclonal antibody (15573-1-AP)

    WB analysis using 15573-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using NDUFC2 Polyclonal antibody (15573-1-AP)

    WB analysis of human liver using 15573-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using NDUFC2 Polyclonal antibody (15573-1-AP)

    WB analysis of human kidney using 15573-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15573-1-AP (NDUFC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFC2 Polyclonal antibody (15573-1-AP)

    IHC staining of human kidney using 15573-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFC2 Polyclonal antibody (15573-1-AP)

    IHC staining of human kidney using 15573-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFC2 Polyclonal antibody (15573-1-AP)

    IHC staining of human kidney using 15573-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NDUFC2 Polyclonal antibody (15573-1-AP)

    IHC staining of human kidney using 15573-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 15573-1-AP (NDUFC2 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using NDUFC2 Polyclonal antibody (15573-1-AP)

    IHC staining of human hepatocirrhosis using 15573-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15573-1-AP (NDUFC2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using NDUFC2 Polyclonal antibody (15573-1-AP)

    IHC staining of human hepatocirrhosis using 15573-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15573-1-AP (NDUFC2 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inA549 cells, human kidney tissue, human liver tissue, Hela cells, HepG2 cells, mouse brain tissue, mouse heart tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 4 publications below

Product Information

15573-1-AP targets NDUFC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NDUFC2 fusion protein Ag7919
Full Name NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC007323
Gene Symbol NDUFC2
Gene ID (NCBI) 4718
RRIDAB_10666733
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NDUFC2 antibody 15573-1-APDownload protocol
IHC protocol for NDUFC2 antibody 15573-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Intellectual disability-associated factor Zbtb11 cooperates with NRF-2/GABP to control mitochondrial function.

Authors - Brooke C Wilson
View Article
pigWB

Sci Rep

Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.

Authors - Liangliang Fu
View Article
humanWB

Cell Rep

A membrane arm of mitochondrial complex I sufficient to promote respirasome formation.

Authors - Hezhi Fang
View Article
mouseWB

Environ Int

Bisphenol S impairs mitochondrial function by targeting Myo19/oxidative phosphorylation pathway contributing to axonal and dendritic injury

Authors - Xing Zhang
View Article