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NTM Polyclonal antibody

NTM Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14373-1-AP

Synonyms

HNT, IgLON family member 2, IGLON2, neurotrimin, NT, NTM, NTRI

Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using NTM Polyclonal antibody (14373-1-AP)

    WB analysis of SH-SY5Y using 14373-1-AP

    WB result of NTM antibody (14373-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NTM transfected SH-SY5Y cells.

  • Western Blot (WB) analysis of SH-SY5Y cells using NTM Polyclonal antibody (14373-1-AP)

    WB analysis of SH-SY5Y using 14373-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14373-1-AP (NTM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using NTM Polyclonal antibody (14373-1-AP)

    WB analysis of mouse brain using 14373-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14373-1-AP (NTM Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NTM Polyclonal antibody (14373-1-AP)

    IHC staining of mouse brain using 14373-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14373-1-AP (NTM antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NTM Polyclonal antibody (14373-1-AP)

    IHC staining of mouse brain using 14373-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14373-1-AP (NTM antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14373-1-AP targets NTM in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NTM fusion protein Ag5746
Full Name neurotrimin
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 58 kDa, 65 kDa
GenBank Accession NumberBC050716
Gene Symbol NTM
Gene ID (NCBI) 50863
RRIDAB_2878048
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neurotrimin (NTM) is a glycosylphosphatidylinositol (GPI)-anchored neural cell adhesion molecule. Neurotrimin together with the limbic system-associated protein (LAMP) and the opioid binding cell adhesion molecule (OBCAM) comprise the IgLON family of the immunoglobulin superfamily. Neurotrimin may be promoting neurite outgrowth and adhesion via a homophilic mechanism.

Protocols

Product Specific Protocols
WB protocol for NTM antibody 14373-1-APDownload protocol
IHC protocol for NTM antibody 14373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols