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BTBD6 Polyclonal antibody

BTBD6 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 13776-1-AP

Synonyms

BDPL, BTB (POZ) domain containing 6, BTBD6, Lens BTB domain protein

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using BTBD6 Polyclonal antibody (13776-1-AP)

    WB analysis of HepG2 using 13776-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13776-1-AP (BTBD6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Y79 cells using BTBD6 Polyclonal antibody (13776-1-AP)

    IP experiment of Y79 using 13776-1-AP

    IP result of anti-BTBD6 (IP:13776-1-AP, 4ug; Detection:13776-1-AP 1:300) with Y79 cells lysate 800ug.

Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inY79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13776-1-AP targets BTBD6 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BTBD6 fusion protein Ag4766
Full Name BTB (POZ) domain containing 6
Calculated Molecular Weight 410 aa, 46 kDa
Observed Molecular Weight 53-58 kDa
GenBank Accession NumberBC042525
Gene Symbol BTBD6
Gene ID (NCBI) 90135
RRIDAB_2877975
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BTBD6 antibody 13776-1-APDownload protocol
IP protocol for BTBD6 antibody 13776-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols