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  • Featured Product
  • KD/KO Validated

SLC25A32 Polyclonal antibody

SLC25A32 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13080-1-AP

Synonyms

MFT, MFTC, SLC25A32

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC25A32 Polyclonal antibody (13080-1-AP)

    WB analysis using 13080-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13080-1-AP (SLC25A32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SLC25A32 Polyclonal antibody (13080-1-AP)

    IHC staining of human prostate cancer using 13080-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13080-1-AP (SLC25A32 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using SLC25A32 Polyclonal antibody (13080-1-AP)

    IHC staining of human prostate cancer using 13080-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13080-1-AP (SLC25A32 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13080-1-AP targets SLC25A32 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A32 fusion protein Ag3722
Full Name solute carrier family 25, member 32
Calculated Molecular Weight 315 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC021893
Gene Symbol SLC25A32
Gene ID (NCBI) 81034
RRIDAB_2877913
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Solute carrier family 25 member 32 (SLC25A32) can transfer tetrahydrofolate (THF) from cellular cytoplasm to the mitochondria as a mitochondrial folate transporter (MFT). It has been reported that SLC25A32 can also transport FAD across the mitochondrial inner membrane. Chronic alcohol exposure affects mitochondrial folate uptake and may affect the transcription of the SLC25A32. And functional SLC25A32 defect is associated with the cranial neural tube defects(PMID: 21956163; 29666258)

Protocols

Product Specific Protocols
WB protocol for SLC25A32 antibody 13080-1-APDownload protocol
IHC protocol for SLC25A32 antibody 13080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

BMC Cancer

SLC25A32 promotes malignant progression of glioblastoma by activating PI3K-AKT signaling pathway

Authors - Zhiwei Xue
  • KD Validated
View Article