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MTCP1NB Polyclonal antibody

MTCP1NB Polyclonal Antibody for IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10235-1-AP

Synonyms

MTCP 1 type A, Mature T-cell proliferation-1 type A, Mature T-cell proliferation 1 neighbor protein, Cx9C motif-containing protein 4, CMC4

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HEK-293T cells using MTCP1NB Polyclonal antibody (10235-1-AP)

    IP experiment of HEK-293T using 10235-1-AP

    IP result of anti-MTCP1NB (IP:10235-1-AP, 4ug; Detection:10235-1-AP 1:300) with HEK-293T cells lysate 1320 ug.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using MTCP1NB Polyclonal antibody (10235-1-AP)

    IF Staining of U2OS using 10235-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MTCP1NB antibody (10235-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive IP detected inHEK-293T cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10235-1-AP targets MTCP1NB in IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MTCP1NB fusion protein Ag0338
Full Name mature T-cell proliferation 1 neighbor
Calculated Molecular Weight 13 kDa, 8 kDa
Observed Molecular Weight 8 kDa
GenBank Accession NumberBC002600
Gene Symbol MTCP1NB
Gene ID (NCBI) 100272147
RRIDAB_2189123
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTCP1NB, also named as C6.1B, MTCP1, MTCP1B, is a member of the CMC4 protein family. MTCP1NB is 8 kDa protein that localizes to mitochondria. MTCP1NB was identified by involvement in some t(X;14) translocations associated with mature T-cell proliferations(NCBI).

Protocols

Product Specific Protocols
IF protocol for MTCP1NB antibody 10235-1-APDownload protocol
IP protocol for MTCP1NB antibody 10235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols