Optimizing Immunoprecipitation of GFP-tagged Proteins in Plant Cell Extracts
This event was held live on August 7th 2025. Watch the on-demand recording.
Immunoprecipitation (IP) is a powerful technique for isolating and analyzing proteins and their interaction partners (co-IP) from complex biological samples. In plant systems, however, the limited availability of antibodies against endogenous plant proteins often leads researchers to use tagged proteins, with green fluorescent protein (GFP) being one of the most widely used tags due to its versatility and ease of detection.
Speaker
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Dr. Mayank Sharma Scientist at ETH Zurich Dr Sharma presented his optimized protocol for IP of GFP-tagged proteins from crude plant cell extracts, as detailed in Methods in Molecular Biology (PMID: 36107351).
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Dr. Sharma covered:
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A step-by-step workflow using GFP-Trap for efficient IP from plant cell extracts
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Practical tips and best practices for maximizing pulldown efficiency
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Troubleshooting guidance to help reduce background and improve reproducibility