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Anticorps Polyclonal de lapin anti-SARS-CoV-2 S protein (126-264 aa)

SARS-CoV-2 S protein (126-264 aa) Polyclonal Antibody for ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Virus

Applications

ELISA

Conjugaison

Non conjugué

N° de cat : 28869-1-AP

Synonymes

SARS-CoV-2 Spike Glycoprotein (126-264 aa), spike, spike protein

Galerie de données de validation

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  • ELISA experiment of Recombinant protein using SARS-CoV-2 S protein (126-264 aa) Polyclonal antib (28869-1-AP)

    ELISA experiment of Recombinant protein using 28869-1-AP

    SARS-CoV-2 Spike Antibody (28869-1-AP) tested by ELISA.SARS-CoV-2 Spike protein was coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (28869-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

Applications testées

Résultats positifs en ELISAProtéine recombinante

Dilution recommandée

ApplicationDilution
Test immuno-enzymatique (ELISA)ELISA :
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

28869-1-AP cible SARS-CoV-2 S protein (126-264 aa) dans les applications de ELISA et montre une réactivité avec des échantillons Virus

Réactivité Virus
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène SARS-CoV-2 S protein (126-264 aa) Protéine recombinante Ag30679
Nom complet SARS-CoV-2 Spike Protein
Masse moléculaire calculée 141 kDa
Numéro d’acquisition GenBankNC_045512
Symbole du gène COVID-19 S Protein
Identification du gène (NCBI) 43740568
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28869-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 126-264aa.