Anticorps Polyclonal de lapin anti-Alpha smooth muscle actin

• Phare
• Validé par KD/KO

Alpha smooth muscle actin Polyclonal Antibody for IF-P

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF-P

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(5)

N° de cat : CL488-14395

Synonymes

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Alpha actin 2

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Galerie de données de validation

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  IF Staining of mouse heart using CL488-14395

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 smooth muscle actin antibody (CL488-14395) at dilution of 1:200.

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  IF Staining of mouse small intestine using CL488-14395

  Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CoraLite® Plus 488 smooth muscle actin antibody (CL488-14395) at dilution of 1:200, CoraLite® Plus 594 ZG16 antibody (CL594-67389, Clone: 1A7B9, red).

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Applications testées

• Résultats positifs en IF-P: tissu cardiaque de souris, tissu d'intestin grêle de souris

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 5 publications below

Informations sur le produit

CL488-14395 cible Alpha smooth muscle actin dans les applications de IF-P et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Alpha smooth muscle actin Protéine recombinante Ag5886
• Nom complet: actin, alpha 2, smooth muscle, aorta
• Masse moléculaire calculée: 377 aa, 42 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC017554
• Symbole du gène: Alpha smooth muscle actin
• Identification du gène (NCBI): 59
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

Publications

Species	Application	Title
mouse	IF	Cell Metab; Myocardial infarction accelerates the progression of MASH by triggering immunoinflammatory response and induction of periosti; Authors - Wei Xie
	IF	Nat Commun; ACSS2 protects against alcohol-induced hepatocyte ferroptosis through regulation of hepcidin expression; Authors - Mengyao Wang
mouse	IF	Front Med; Targeting deubiquitinase OTUB1 protects vascular smooth muscle cells in atherosclerosis by modulating PDGFRβ; Authors - Fei Xu
	IF	Diabet Med; Mouse islet-derived stellate cells are similar to, but distinct from, mesenchymal stromal cells and influence the beta cell function; Authors - Wei Xu
rat	IF	Regen Biomater; Magnetic fibrin nanofiber hydrogel delivering iron oxide magnetic nanoparticles promotes peripheral nerve regeneration; Authors - Juncong Hong

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