Anticorps Polyclonal de lapin anti-Phospho-Marcksl1

Phospho-Marcksl1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (3)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10018-3-AP

Synonymes

MARCKS, MARCKS-like 1, PKCSL, Myristoylated alanine-rich C-kinase substrate, Marcksl1

---

Galerie de données de validation

• 

  WB analysis using 10018-3-AP

  Non-treated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10018-3-AP (Phospho-Marcksl1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

---

Applications testées

• Résultats positifs en WB: cellules RAW 264.7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

10018-3-AP cible Phospho-Marcksl1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: rat, bovin, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myristoylated alanine rich protein kinase C substrate
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC046601
• Symbole du gène: Marcks
• Identification du gène (NCBI): 17118
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. This antibody is raised against phospho-Marcksl1 (geneID:17357) with S93/S104 phosphorylated. Because of the sequence similarity, this antibody can recognize phospho-Marcksl1 and phospho-Marcks.

Publications

Species	Application	Title
bovine	WB	Proc Natl Acad Sci U S A; MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability.; Authors - Jin Benjamin Y BY
rat	WB	J Neurosci; NADPH oxidase is required for the sensory plasticity of the carotid body by chronic intermittent hypoxia.; Authors - Peng Y-J YJ
rat	WB	J Physiol; 5-HT evokes sensory long-term facilitation of rodent carotid body via activation of NADPH oxidase.; Authors - Peng Ying-Jie YJ
rat	WB	Am J Physiol Cell Physiol; Involvement of PKCdelta and PKD in pulmonary microvascular endothelial cell hyperpermeability.; Authors - Tinsley John H JH
human	WB	Open Virol J; HIV-1 Antigens in Neurons of Cocaine-Abusing Patients.; Authors - Fiala Milan M