Anticorps Polyclonal de lapin anti-Pan-PAX

Pan-PAX Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 21383-1-AP

Synonymes

paired box, paired box 8, Paired box protein Pax, Paired box protein Pax 8, Pan-PAX, PAX 8

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Galerie de données de validation

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  WB analysis of SKOV-3 using 21383-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 21383-1-AP (Pan-PAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 21383-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 21383-1-AP (Pan-PAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of SKOV-3 using 21383-1-AP

  IP result of anti-Pan-PAX (IP:21383-1-AP, 3ug; Detection:21383-1-AP 1:800) with SKOV-3 cells lysate 2500ug.

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  IHC staining of human ovary tumor using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 21383-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 21383-1-AP (Pan-PAX antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SKOV-3 using 21383-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using Pan-PAX antibody (21383-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules SKOV-3, cellules A2780
• Résultats positifs en IP: cellules SKOV-3
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de carcinome à cellules rénales humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SKOV-3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

21383-1-AP cible Pan-PAX dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Pan-PAX Protéine recombinante Ag16018
• Nom complet: paired box 8
• Masse moléculaire calculée: 450 aa, 48 kDa
• Poids moléculaire observé: 36-48 kDa
• Numéro d’acquisition GenBank: BC001060
• Symbole du gène: PAX8
• Identification du gène (NCBI): 7849
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAX genes encode a family of developmentally regulated transcription factors that have been implicated in a number of human and murine congenital disorders, as well as in tumorigenesis. These genes are defined by the presence of an evolutionarily conserved DNA binding domain, termed the paired domain. Many paired domain-containing proteins recognize a common sequence motif. The immunogen of this atnibody is the Paired box domain of PAX family. This antibody is a Pan-PAX antibody.

Publications

Species	Application	Title
mouse	IF	Life Sci; Notch-mediated Sox9+ cell activation contributes to kidney repair after partial nephrectomy.; Authors - Qiwang Ma
rat	IHC	Sci Signal; Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn.; Authors - Zi-Yang Zhang
human	IHC	Am J Surg Pathol; PAX8 and PAX2 immunostaining facilitates the diagnosis of primary epithelial neoplasms of the male genital tract.; Authors - Tong Guo-Xia GX
mouse	IHC	Endocrine; PMID: 32281047; Authors - Mika Shimamura
pig	IF	PLoS One; PMID: 28231273; Authors - Sergio E Palma-Vera