Anticorps Polyclonal de lapin anti-nNOS

nNOS Polyclonal Antibody for IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

IHC, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 18984-1-AP

Synonymes

bNOS, Constitutive NOS, IHPS1, N NOS, NC NOS, Neuronal NOS, Nitric oxide synthase, brain, nNOS, NOS, NOS type I, NOS1

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Galerie de données de validation

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  IHC staining of mouse heart using 18984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 18984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 18984-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 18984-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 18984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 18984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18984-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18984-1-AP (nNOS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en IHC: tissu cardiaque de souris, tissu cérébral de souris, tissu rénal de souris, tissu testiculaire de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below

Informations sur le produit

18984-1-AP cible nNOS dans les applications de IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nitric oxide synthase 1 (neuronal)
• Masse moléculaire calculée: 161 kDa
• Numéro d’acquisition GenBank: NM_000620
• Symbole du gène: nNOS
• Identification du gène (NCBI): 4842
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

nNOS belongs to the NOS family. nNOS produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. NO is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. NO is synthesized from L-arginine by nitric oxide synthases. nNOS is a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in nNOS gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1).The antibody can recognize isoform 1,2,4 of nNOS.

Publications

Species	Application	Title
mouse		ACS Chem Neurosci; Antidepressant-like Effect of Merazin Hydrate Depends on NO/ERK by Suppressing Its Downstream NF-κB or Nonactivating CREB/BDNF in Mouse Hippocampus.; Authors - Lei Wu
canine	IHC	Exp Eye Res; Altered transsulfuration pathway enzymes and redox homeostasis in inherited retinal degenerative diseases.; Authors - Alireza Badiei
rat		Acta Pharmacol Sin; Neuroprotective effects of scutellarin on rat neuronal damage induced by cerebral ischemia/reperfusion.; Authors - Hu Xia-Min XM
mouse		Biochim Biophys Acta Mol Cell Res; NMDA receptor activation induces damage of alveolar type II cells and lung fibrogenesis through ferroptosis; Authors - Hai-Peng Cheng
pig	IHC	Eur J Pharmacol; Suppressive effect of nitric oxide synthase (NOS) inhibitor L-NMMA acetate on choroidal fibrosis in experimental myopic guinea pigs through the nitric oxide signaling pathway; Authors - Tuling Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Reyes (Verified Customer) (03-01-2024)

Used nNOS antibody on human cortex brain sections (in red), it worked very nicely

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

nNOS Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:18984-1-AP)