Anticorps Polyclonal de lapin anti-Hamartin

• Phare
• Validé par KD/KO

Hamartin Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 20988-1-AP

Synonymes

Hamartin, KIAA0243, LAM, TSC, TSC1, tuberous sclerosis 1, Tuberous sclerosis 1 protein

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Galerie de données de validation

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  WB analysis of HeLa using 20988-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20988-1-AP (hamartin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20988-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20988-1-AP (hamartin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 20988-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 20988-1-AP (hamartin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 20988-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 20988-1-AP (hamartin antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 20988-1-AP

  Immunofluorescent analysis of HeLa cells using 20988-1-AP (hamartin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

20988-1-AP cible Hamartin dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tuberous sclerosis 1
• Masse moléculaire calculée: 130 kDa
• Numéro d’acquisition GenBank: NM_000368
• Symbole du gène: TSC1
• Identification du gène (NCBI): 7248
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSC1, also named as KIAA0243 and TSC, is implicated as a tumor suppressor. It is involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. In complex with TSC2, TSC1 inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. It seems not to be required for TSC2 GAP activity towards RHEB. It is involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Defects in TSC1 are the cause of tuberous sclerosis complex (TSC). Defects in TSC1 may be a cause of focal cortical dysplasia of Taylor balloon cell type (FCDBC). The antibody is specific to TSC1. This antibody can recognize two isoforms: 70 kDa (667aa) and 130 kDa.

Publications

Species	Application	Title
human	WB	Sci Adv; Synthetic lethality by targeting the RUVBL1/2-TTT complex in mTORC1-hyperactive cancer cells.; Authors - Seung Ho Shin
mouse	WB	Theranostics; Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief.; Authors - Jiqian Zhang ; KD Validated
human	WB	Mol Ther Nucleic Acids; miR-301a Suppression within Fibroblasts Limits the Progression of Fibrosis through the TSC1/mTOR Pathway.; Authors - Jiexuan Wang ; KD Validated
mouse	WB	Sci Rep; Tsc1 deficiency impairs mammary development in mice by suppression of AKT, nuclear ERα, and cell-cycle-driving proteins.; Authors - Zhenqi Qin ; KO Validated
mouse	WB	FASEB J; mTORC1 promotes mineralization via p53 pathway.; Authors - Xinghong Luo
human	WB,IHC,IP	Biochem Biophys Res Commun; UBE2S exerts oncogenic activities in urinary bladder cancer by ubiquitinating TSC1.; Authors - Hao Tang ; KD Validated