Anticorps Polyclonal de lapin anti-Beta Catenin

• Phare
• Validé par KD/KO

Beta Catenin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

113

N° de cat : 17565-1-AP

Synonymes

CTNNB, Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin

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Galerie de données de validation

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  WB analysis using 17565-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17565-1-AP (Beta Catenin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17565-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 17565-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17565-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of NIH/3T3 using 17565-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17565-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17565-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of A431 using 17565-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of MCF-7 using 17565-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17565-1-AP (beta-Catenin antibody at dilution of 1:600 incubated at 4 degree celsius over night.

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  IP experiment of HEK-293 using 17565-1-AP

  IP result of anti-Beta Catenin (IP:17565-1-AP, 4ug; Detection:17565-1-AP 1:5000) with HEK-293 cells lysate 1920 ug.

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  IHC staining of human liver cancer using 17565-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17565-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 17565-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17565-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17565-1-AP (beta-Catenin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 17565-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of MCF-7 using 17565-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, green).

• 

  IF Staining of MCF-7 using 17565-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, green).

• 

  IF Staining of MCF-7 using 17565-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of T-47D using 17565-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using Beta Catenin antibody (17565-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  FC experiment of MCF-7 using 17565-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Beta Catenin (17565-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules C6, cellules HeLa, cellules MCF-7, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HEK-293,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7, cellules T-47D, cellules U-251
• Résultats positifs en FC (Intra): cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 92 publications below
• IHC: See 18 publications below
• IF: See 31 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

17565-1-AP cible Beta Catenin dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Beta Catenin Protéine recombinante Ag11763
• Nom complet: catenin (cadherin-associated protein), beta 1, 88kDa
• Masse moléculaire calculée: 781 aa, 86 kDa
• Poids moléculaire observé: 92 kDa
• Numéro d’acquisition GenBank: BC058926
• Symbole du gène: Beta Catenin
• Identification du gène (NCBI): 1499
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

Publications

Species	Application	Title
mouse	WB,IF	J Clin Invest; JAM3 maintains leukemia-initiating cell self-renewal through LRP5/AKT/β-catenin/CCND1 signaling.; Authors - Yaping Zhang
human	WB,IHC,IF,IP	Cell Death Dis; USP8 positively regulates hepatocellular carcinoma tumorigenesis and confers ferroptosis resistance through β-catenin stabilization; Authors - Jianing Tang
rat	WB,IF	Mater Today Bio; A novel adhesive dual-sensitive hydrogel for sustained release of exosomes derived from M2 macrophages promotes repair of bone defects; Authors - Xiaoying Zhao
human	IHC,IF	Br J Pharmacol; Reregulation of hepatic stellate cell contraction and cirrhotic portal hypertension by Wnt/β-catenin signaling via interaction with Gli1.; Authors - Feng Zhang
human	WB	Acta Pharmacol Sin; Identification of HMGCR as the anticancer target of physapubenolide against melanoma cells by in silico target prediction.; Authors - Hai-Yan Wang
human		Int J Nanomedicine; Self-Assembly Of Retinoid Nanoparticles For Melanoma Therapy.; Authors - Han Liao