Anticorps Polyclonal de lapin anti-ZW10

• Phare
• Validé par KD/KO

ZW10 Polyclonal Antibody for WB, IP, IF, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 24561-1-AP

Synonymes

HZW10

---

Galerie de données de validation

• 

  WB analysis using 24561-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24561-1-AP (ZW10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24561-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24561-1-AP (ZW10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24561-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24561-1-AP (ZW10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 24561-1-AP

  IP result of anti-ZW10 (IP:24561-1-AP, 4ug; Detection:24561-1-AP 1:500) with Jurkat cells lysate 3200ug.

• 

  IF Staining of HeLa using 24561-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ZW10 antibody (24561-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 24561-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ZW10 (24561-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules K-562, tissu hépatique de rat, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

24561-1-AP cible ZW10 dans les applications de WB, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZW10 Protéine recombinante Ag19138
• Nom complet: ZW10, kinetochore associated, homolog (Drosophila)
• Masse moléculaire calculée: 779 aa, 89 kDa
• Poids moléculaire observé: 88-90 kDa
• Numéro d’acquisition GenBank: BC128264
• Symbole du gène: ZW10
• Identification du gène (NCBI): 9183
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZW10 is the human homolog of the Drosophila melanogaster Zw10 protein. ZW10 is localized to kinetochores and kinetochore-associated microtubules and is an essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis(PMID: 17102640). ZW10 proteins in Drosophila and HeLa cells display a similar and intriguing cell cycle-dependent intracellular distribution. ZW10 protein first becomes localized to the kinetochore at prometaphase, but then appears to move onto the kinetochore microtubules of the spindle at metaphase, and then back to the kinetochore at anaphase(PMID: 9700164).

Publications

Species	Application	Title
human	WB,IF	J Clin Invest; RINT1 deficiency disrupts lipid metabolism and underlies a complex hereditary spastic paraplegia; Authors - Nathalie Launay
human	IF	Nat Commun; Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.; Authors - Haining Zhou
human	WB	Genes Dev; Functional dissection of human mitotic genes using CRISPR-Cas9 tiling screens.; Authors - Jacob A Herman
mouse	WB	J Cell Biol; Rab18 promotes lipid droplet (LD) growth by tethering the ER to LDs through SNARE and NRZ interactions.; Authors - Dijin Xu
human,monkey	WB	Cell Rep; The ER-Localized Protein DFCP1 Modulates ER-Lipid Droplet Contact Formation.; Authors - Dongfang Li ; KD Validated
human	WB	iScience; TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment; Authors - Magdalena Wolczyk