Anticorps Polyclonal de lapin anti-ZNF746

• Phare
• Validé par KD/KO

ZNF746 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 24543-1-AP

Synonymes

PARIS, zinc finger protein 746, ZNF746

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Galerie de données de validation

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  WB analysis using 24543-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24543-1-AP (ZNF746 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 24543-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 24543-1-AP (ZNF746 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HEK-293T

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

24543-1-AP cible ZNF746 dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZNF746 Protéine recombinante Ag21566
• Nom complet: zinc finger protein 746
• Masse moléculaire calculée: 644 aa, 69 kDa
• Poids moléculaire observé: 85-95 kDa
• Numéro d’acquisition GenBank: BC068505
• Symbole du gène: ZNF746
• Identification du gène (NCBI): 155061
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZNF746, also named as PARIS, which belongs to the krueppel C2H2-type zinc-finger protein family. ZNF746 mainly localizes to the cytoplasm and probably translocates to the nucleus to repress selected genes. ZNF746 as a transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death. ZNF746 may act as a downstream effector of PRKN and contribute to neurodegeneration in Parkinson disease cases caused by defects in PRKN (PMID: 28122242). The calculated molecular weight of ZNF746 is 69 kDa, but Phosphorylation of ZNF746 is about 80-85 kDa (PMID: 28122242).

Publications

Species	Application	Title
mouse	WB	Neuron; Neuronal NLRP3 is a parkin substrate that drives neurodegeneration in Parkinson's disease.; Authors - Nikhil Panicker ; KO Validated
human,mouse	WB	Sci Adv; Deubiquitinase CYLD acts as a negative regulator of dopamine neuron survival in Parkinson's disease.; Authors - Sheila K Pirooznia
mouse	WB	Brain; Parkin interacting substrate zinc finger protein 746 is a pathological mediator in Parkinson's disease.; Authors - Saurav Brahmachari
human,mouse	WB,IF	Brain; Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration.; Authors - Hyojung Kim
mouse	WB	Front Aging Neurosci; Identification and evaluation of midbrain specific longevity-related genes in exceptionally long-lived but healthy mice; Authors - Hyojung Kim
human,mouse	WB,IHC	Biochem Biophys Res Commun; ZNF746/PARIS promotes the occurrence of hepatocellular carcinoma.; Authors - Hanna Kim ; KO Validated