Anticorps Polyclonal de lapin anti-ZNF238/ZBTB18

• Phare
• Validé par KD/KO

ZNF238/ZBTB18 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, RIP, IHC, CoIP, chIP, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 12714-1-AP

Synonymes

58 kDa repressor protein, C2H2 171, RP58, TAZ 1, TAZ1, Transcriptional repressor RP58, ZBTB18, ZFP238, zinc finger protein 238, Zinc finger protein C2H2 171, ZNF238

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Galerie de données de validation

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  WB analysis using 12714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12714-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12714-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12714-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12714-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12714-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12714-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 12714-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12714-1-AP (ZNF238 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 12714-1-AP

  IP result of anti-ZNF238/ZBTB18 (IP:12714-1-AP, 4ug; Detection:12714-1-AP 1:3000) with rat brain tissue lysate 1880 ug.

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  IHC staining of mouse brain using 12714-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12714-1-AP (ZNF238 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 12714-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12714-1-AP (ZNF238 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse skin using 12714-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12714-1-AP (ZNF238 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 12714-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12714-1-AP (ZNF238 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de cervelet de souris, tissu cardiaque de rat, tissu cardiaque de souris, tissu cérébral de souris, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de rat,
• Résultats positifs en IHC: tissu cérébral de souris, tissu cutané de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• CoIP: See 2 publications below
• ChIP: See 3 publications below
• RIP: See 1 publications below

Informations sur le produit

12714-1-AP cible ZNF238/ZBTB18 dans les applications de WB, IP, IF, RIP, IHC, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZNF238/ZBTB18 Protéine recombinante Ag3406
• Nom complet: zinc finger protein 238
• Masse moléculaire calculée: 531 aa, 59 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC036677
• Symbole du gène: ZNF238/ZBTB18
• Identification du gène (NCBI): 10472
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZNF238 is a member of the BTB/POZ-ZF protein family, which involve in development and cancer formation, for example BCL-6, PLZF, and HIC-1. It's a transcriptional repressor involve in myogenesis and brain development. By directly repressing the expression of two skeletal myogenesis inhibitors, ID2 and ID3, ZNF238 plays a key role in myogenesis. It can control cell division of progenitor cells and regulating the survival of postmitotic cortical neurons. Besides, ZNF238 involves in the organization of nuclear chromosomes, for its specific binding to the consensus DNA sequence that contains the E box core, and recruiting chromatin remodeling multi-protein complex. ZNF238 proteins has apparent molecular masses of 60 and 48 kD. Specific binding is found for a 60-kDa band which corresponds to the full length of RP58 protein. In addition, a 48-kDa band, thought to be the truncated form 2 is detected (PMID: 9756912).

Publications

Species	Application	Title
mouse	chIP	Neuron; FOXG1 Orchestrates Neocortical Organization and Cortico-Cortical Connections.; Authors - Francesca Cargnin
mouse	IF	PLoS Biol; Olig2 and Hes regulatory dynamics during motor neuron differentiation revealed by single cell transcriptomics.; Authors - Andreas Sagner
mouse	IHC	Cereb Cortex; The Zinc Finger Transcription Factor RP58 Negatively Regulates Rnd2 for the Control of Neuronal Migration During Cerebral Cortical Development.; Authors - Heng Julian Ik-Tsen JI
human	WB,CoIP	iScience; Calpain-mediated cleavage generates a ZBTB18 N-terminal product that regulates HIF1A signaling and glioblastoma metabolism.; Authors - Anie P Masilamani
MOUSE	IF	Neural Dev; Rp58 and p27(kip1) coordinate cell cycle exit and neuronal migration within the embryonic mouse cerebral cortex.; Authors - Olivier Clément
human	WB,RIP	MedComm (2020); LINC02774 inhibits glycolysis in glioma to destabilize HIF-1α dependent on transcription factor RP58; Authors - Yuanbing Chen