Anticorps Polyclonal de lapin anti-ZNF23

ZNF23 Polyclonal Antibody for WB, IP, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 21322-1-AP

Synonymes

KOX16, Zfp612, Zinc finger protein 23, Zinc finger protein 359, Zinc finger protein KOX16, ZNF23, ZNF359, ZNF612

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Galerie de données de validation

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  WB analysis of HepG2 using 21322-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21322-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21322-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A2780 using 21322-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 21322-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 21322-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 21322-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21322-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21322-1-AP (ZNF23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 21322-1-AP

  IP result of anti-ZNF23 (IP:21322-1-AP, 4ug; Detection:21322-1-AP 1:800) with mouse brain tissue lysate 4000ug.

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  IF Staining of HepG2 using 21322-1-AP

  Immunofluorescent analysis of HepG2 cells, using ZNF23 antibody 21322-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules A2780, cellules HeLa, cellules SKOV-3, tissu cérébral de souris, tissu pulmonaire de souris, tissu splénique de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

21322-1-AP cible ZNF23 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZNF23 Protéine recombinante Ag14755
• Nom complet: zinc finger protein 23 (KOX 16)
• Masse moléculaire calculée: 643 aa, 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC007256
• Symbole du gène: ZNF23
• Identification du gène (NCBI): 7571
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC	Cell Physiol Biochem; ZNF23 Suppresses Cutaneous Melanoma Cell Malignancy via Mitochondria-Dependent Pathway.; Authors - Xin Zhang