Anticorps Polyclonal de lapin anti-ZNF143

• Phare
• Validé par KD/KO

ZNF143 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 16618-1-AP

Synonymes

hStaf, pHZ 1, SBF, SPH binding factor, STAF, zinc finger protein 143, ZNF143

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Galerie de données de validation

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  WB analysis of HepG2 using 16618-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16618-1-AP (ZNF143 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16618-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16618-1-AP (ZNF143 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 16618-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16618-1-AP (ZNF143 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 8 publications below

Informations sur le produit

16618-1-AP cible ZNF143 dans les applications de WB, IP, IF, IHC, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZNF143 Protéine recombinante Ag9914
• Nom complet: zinc finger protein 143
• Masse moléculaire calculée: 626 aa, 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC020219
• Symbole du gène: ZNF143
• Identification du gène (NCBI): 7702
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Zinc finger protein143(ZNF143) contains a separete acidic activation and 7 zinc finger domains. It's a transcription factor that required for activation of the majority of vertebrate snRNA and snRNA-type genes, which transcribed by RNA pol II and pol III. Also, it binds to the SPH motif of small nuclear RNA (snRNA) gene promoters and participates in efficient U6 RNA polymerase III transcription via its interaction with CHD8

Publications

Species	Application	Title
mouse	chIP	Mol Cell; Maintenance of CTCF- and Transcription Factor-Mediated Interactions from the Gametes to the Early Mouse Embryo.; Authors - Yoon Hee Jung
human	chIP	Mol Cell; Architectural Proteins and Pluripotency Factors Cooperate to Orchestrate the Transcriptional Response of hESCs to Temperature Stress.; Authors - Xiaowen Lyu
mouse	CoIP	Nat Commun; Mutations in Hcfc1 and Ronin result in an inborn error of cobalamin metabolism and ribosomopathy.; Authors - Tiffany Chern
human	ChIP	Proc Natl Acad Sci U S A; Cooperative transcription factor associations discovered using regulatory variation.; Authors - Karczewski Konrad J KJ
human	chIP	Cancer Res; ZNF143-Mediated H3K9 Trimethylation Upregulates CDC6 by Activating MDIG in Hepatocellular Carcinoma.; Authors - Lili Zhang ; KD Validated
human	WB, ChIP	Cell Rep; Host Cell Factor-1 Recruitment to E2F-Bound and Cell-Cycle-Control Genes Is Mediated by THAP11 and ZNF143.; Authors - J Brandon Parker ; KD Validated