Anticorps Polyclonal de lapin anti-ZMYND8

• Phare
• Validé par KD/KO

ZMYND8 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 11633-1-AP

Synonymes

CTCL associated antigen se14 3, KIAA1125, PRKCBP1, PRO2893, RACK7, ZMYND8

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Galerie de données de validation

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  WB analysis of MCF-7 using 11633-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11633-1-AP (ZMYND8 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 11633-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11633-1-AP (ZMYND8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11633-1-AP

  IP result of anti-ZMYND8 (IP:11633-1-AP, 3ug; Detection:11633-1-AP 1:300) with HeLa cells lysate 920ug.

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  IHC staining of human lung cancer using 11633-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11633-1-AP (ZMYND8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11633-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11633-1-AP (ZMYND8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11633-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11633-1-AP (ZMYND8 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11633-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11633-1-AP (ZMYND8 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

11633-1-AP cible ZMYND8 dans les applications de WB, IP, IF, IHC, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZMYND8 Protéine recombinante Ag2207
• Nom complet: zinc finger, MYND-type containing 8
• Masse moléculaire calculée: 1186 aa, 132 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC030721
• Symbole du gène: ZMYND8
• Identification du gène (NCBI): 23613
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Regulation of neuronal gene expression is essential to nervous system development. REST (RE1-silencing transcription factor) regulates neuronal gene expression by interacting with a group of corepressor proteins including REST corepressors (RCOR). ZMYND8 bind to ROCR2 and can function as transcriptional repressors. It is co-expressed with RCOR2 in the nervous system and overexpression of ZMYND8 inhibits neural differentiation. [PMID:20331974]

Publications

Species	Application	Title
mouse,human		Mol Cell; The ZMYND8-regulated mevalonate pathway endows YAP-high intestinal cancer with metabolic vulnerability.; Authors - Qiang Pan ; KO Validated
human	WB	Mol Cancer; LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.; Authors - Xi Jin ; KD Validated
human	WB	Cancer Res; KDM5C-mediated recruitment of BRD4 to chromatin regulates enhancer activation and BET inhibitor sensitivity; Authors - Yulong Qiang
human	WB,IHC,IF	Sci Adv; The endogenous retrovirus-derived long noncoding RNA TROJAN promotes triple-negative breast cancer progression via ZMYND8 degradation.; Authors - Xi Jin
mouse	ChIP	J Tissue Eng; Mesenchymal stem cells overexpressing XIST induce macrophage M2 polarization and improve neural stem cell homeostatic microenvironment, alleviating spinal cord injury; Authors - Dan Zhu