Anticorps Polyclonal de lapin anti-ZHX2

• Phare
• Validé par KD/KO

ZHX2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 20136-1-AP

Synonymes

AFP regulator 1, AFR1, Alpha fetoprotein regulator 1, KIAA0854, RAF, Regulator of AFP, ZHX2, zinc fingers and homeoboxes 2

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Galerie de données de validation

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  WB analysis using 20136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20136-1-AP (ZHX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20136-1-AP

  WB result of ZHX2 antibody (20136-1-AP; 1:24000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZHX2 transfected HEK-293 cells.

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  WB analysis of mouse skeletal muscle using 20136-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20136-1-AP (ZHX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 20136-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20136-1-AP (ZHX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 20136-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20136-1-AP (ZHX2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20136-1-AP (ZHX2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 20136-1-AP

  IP result of anti-ZHX2 (IP:20136-1-AP, 4ug; Detection:20136-1-AP 1:800) with mouse brain tissue lysate 2800ug.

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  IHC staining of human colon cancer using 20136-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20136-1-AP (ZHX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20136-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20136-1-AP (ZHX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 20136-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ZHX2 antibody (20136-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris, tissu de muscle squelettique de souris, tissu ovarien de souris, tissu pulmonaire de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

20136-1-AP cible ZHX2 dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZHX2 Protéine recombinante Ag14088
• Nom complet: zinc fingers and homeoboxes 2
• Masse moléculaire calculée: 837 aa, 92 kDa
• Poids moléculaire observé: 92-100 kDa
• Numéro d’acquisition GenBank: BC042145
• Symbole du gène: ZHX2
• Identification du gène (NCBI): 22882
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Zinc fingers and homeoboxes protein 2 is a protein that in humans is encoded by the ZHX2 gene. ZHX2 Act as a transcriptional repressor, represses the promoter activity of the CDC25C gene stimulated by NFYA (PMID:12741956). In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.

Publications

Species	Application	Title
human	WB	Int J Cancer; HBV suppresses ZHX2 expression to promote proliferation of HCC through miR-155 activation.; Authors - Xiaojia Song
human	WB	J Pathol; ZHX2 inhibits SREBP1c-mediated de novo lipogenesis in hepatocellular carcinoma via miR-24-3p.; Authors - Xiangguo Yu
mouse	WB	J Immunol; Zhx2 Accelerates Sepsis by Promoting Macrophage Glycolysis via Pfkfb3.; Authors - Zehua Wang ; KO Validated
human	WB,IHC	Cancer Cell Int; ZHX2 inhibits thyroid cancer metastasis through transcriptional inhibition of S100A14.; Authors - Yankun Zhang ; KD Validated
human	WB	Biochem Biophys Res Commun; Proliferation and osteo/odontogenic differentiation of stem cells from apical papilla regulated by Zinc fingers and homeoboxes 2: An in vitro study.; Authors - Fang Wan ; KD Validated
human	WB,IHC	FEBS Open Bio; Upregulation of ZHX2 predicts poor prognosis and is correlated with immune infiltration in gastric cancer.; Authors - Anqi Cheng