Anticorps Polyclonal de lapin anti-Protrudin

• Phare
• Validé par KD/KO

Protrudin Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 12680-1-AP

Synonymes

PROTRUDIN, RP11 459F3.2, SPG33, ZFYVE27

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Galerie de données de validation

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  WB analysis of mouse thymus using 12680-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12680-1-AP (Protrudin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12680-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12680-1-AP (Protrudin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12680-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12680-1-AP (Protrudin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12680-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12680-1-AP (Protrudin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu de thymus de souris, cellules Jurkat, tissu cérébral de souris, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IF: See 4 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

12680-1-AP cible Protrudin dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protrudin Protéine recombinante Ag3369
• Nom complet: zinc finger, FYVE domain containing 27
• Masse moléculaire calculée: 411 aa, 46 kDa
• Poids moléculaire observé: 46-50 kDa
• Numéro d’acquisition GenBank: BC030621
• Symbole du gène: ZFYVE27
• Identification du gène (NCBI): 118813
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protrudin, also named as ZFYVE27, is a 411 amino acid protein, which contains 1 FYVE-type zinc finger. Protrudin localizes in the recycling endosome membrane and functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. Protrudin is a key regulator of vesicular transport during neurite extension and is involved in neuronal intracellular trafficking in the corticospinal tract.

Publications

Species	Application	Title
human	WB,IP	Nat Commun; PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria.; Authors - Yael Elbaz-Alon
rat	WB	Nat Commun; Protrudin functions from the endoplasmic reticulum to support axon regeneration in the adult CNS.; Authors - Veselina Petrova
human	WB	Nat Commun; Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry.; Authors - Parikshit Bagchi
human	WB,IF,IP	Proc Natl Acad Sci U S A; Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.; Authors - Chang Jaerak J ; KD Validated
human		Cell Mol Life Sci; ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic.; Authors - James W Connell
human	WB	Cell Mol Life Sci; Protrudin regulates FAK activation, endothelial cell migration and angiogenesis.; Authors - Amita Arora ; KD Validated