Anticorps Polyclonal de lapin anti-Rabenosyn 5

Rabenosyn 5 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 22218-1-AP

Synonymes

110 kDa protein, Rabenosyn 5, ZFYVE20

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Galerie de données de validation

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  WB analysis of K-562 using 22218-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 22218-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 22218-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 22218-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 22218-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22218-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 22218-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 22218-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 22218-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 22218-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 22218-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 22218-1-AP (Rabenosyn 5 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules A549, tissu cardiaque de souris, tissu cérébral de souris, tissu de muscle squelettique de souris, tissu rénal de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

22218-1-AP cible Rabenosyn 5 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Rabenosyn 5 Protéine recombinante Ag17554
• Nom complet: zinc finger, FYVE domain containing 20
• Masse moléculaire calculée: 784 aa, 89 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC106940
• Symbole du gène: ZFYVE20
• Identification du gène (NCBI): 64145
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	EMBO Rep; Amino acid starvation-induced LDLR trafficking accelerates lipoprotein endocytosis and LDL clearance.; Authors - Ye Chen
human	WB, IHC	J Mol Biol; Identification of New Interactions between Endolysosomal Tethering Factors.; Authors - Zsófia Simon-Vecsei
mouse	WB,IF,IP	Int J Mol Sci; Poly(ADP-ribose) Polymerase 1 Mediates Rab5 Inactivation after DNA Damage; Authors - Masato Mashimo