Anticorps Monoclonal anti-ZEB2

ZEB2 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

CloneNo.

1D3A2

N° de cat : 67514-1-Ig

Synonymes

FLJ42816, KIAA0569, SIP 1, SIP1, Smad interacting protein 1, SMADIP1, ZEB2, ZFHX1B, ZFX1B

---

Galerie de données de validation

• 

  WB analysis using 67514-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67514-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 67514-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67514-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67514-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67514-1-Ig (ZEB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67514-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67514-1-Ig (ZEB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67514-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67514-1-Ig (ZEB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules K-562, cellules 4T1, cellules HSC-T6, cellules HT-1080, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

67514-1-Ig cible ZEB2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ZEB2 Protéine recombinante Ag25477
• Nom complet: zinc finger E-box binding homeobox 2
• Poids moléculaire observé: 160 kDa
• Numéro d’acquisition GenBank: BC127102
• Symbole du gène: ZEB2
• Identification du gène (NCBI): 9839
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Zinc finger E-box-binding homeobox (ZEB2) is a key transcription factor that acts as a multifunctional regulator during nervous system development. ZEB2 contains two zinc finger domains and a homeodomain-like sequence and interacts with the TGF-ß superfamily signaling regulators, Smads, to regulate the expression of their downstream genes. ZEB2 is expressed in the developing neural tube, as well as in neural crest cells, the hippocampus and the cerebral cortex. ZEB2 has previously been implicated in EMT, cell-cycle progression, apoptosis and senescence. ZEB2 was overexpressed in bladder, ovarian, stomach, pancreatic and squamous cell carcinoma, in the intestinal subtype of stomach cancers, and at the invasive front of CRC where EMT is most prominen. ZEB2 also mediates cell-fate decision in neuronal, T cells and hematopoietic stem cells. The calculated molecular weight of ZEB2 is 136 kDa, but we find the 95 kDa band in some publication(PMID: 27659015 )

Publications

Species	Application	Title
human	WB	J Med Chem; Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis.; Authors - Ting Peng
human	WB	Exp Cell Res; TRIM16 overexpression inhibits the metastasis of colorectal cancer through mediating Snail degradation.; Authors - Longhui Ruan
human	WB	Exp Physiol; ZEB1 recruit Brg1 to regulate airway remodeling epithelial-mesenchymal transition in asthma.; Authors - Ting Wang
human	WB,IF	Int J Med Sci; Lnc NR2F1-AS1 Promotes Breast Cancer Metastasis by Targeting the MiR-25-3p/ZEB2 Axis; Authors - Duanyang Zhai ; KD Validated