Anticorps Polyclonal de lapin anti-ZDHHC9

ZDHHC9 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 24046-1-AP

Synonymes

CGI 89, CXorf11, DHHC 9, DHHC9, MMSA 1, Palmitoyltransferase ZDHHC9, UNQ261/PRO298, ZDHHC10, ZDHHC9, Zinc finger protein 379, ZNF379, ZNF380

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Galerie de données de validation

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  WB analysis using 24046-1-AP

  Mouse liver tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24046-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 24046-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 24046-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 24046-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 24046-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 24046-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 24046-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24046-1-AP (ZDHHC9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 24046-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24046-1-AP (ZDHHC9 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of SH-SY5Y using 24046-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 24046-1-AP (ZDHHC9 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules SH-SY5Y, tissu cérébral de souris, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

24046-1-AP cible ZDHHC9 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZDHHC9 Protéine recombinante Ag20881
• Nom complet: zinc finger, DHHC-type containing 9
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 38-48 kDa
• Numéro d’acquisition GenBank: BC006200
• Symbole du gène: ZDHHC9
• Identification du gène (NCBI): 51114
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZDHHC9, also named as CXorf11, ZDHHC10, ZNF379 or ZNF380, is a 364 amino acid protein, which contains one DHHC-type zinc finger and belongs to the DHHC palmitoyltransferase activity. ZDHHC9 localizes in the endoplasmic reticulum membrane and is highly expressed in kidney, skeletal muscle, brain, lung and liver. The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS.

Publications

Species	Application	Title
human,mouse	IHC,IF	Discov Oncol; ZDHHC9: a promising therapeutic target for triple-negative breast cancer through immune modulation and immune checkpoint blockade resistance; Authors - Chao Xu
human	WB	Cancer Lett; Palmitoylation alters LDHA activity and pancreatic cancer response to chemotherapy; Authors - Luojun Chen