Anticorps Polyclonal de lapin anti-ZCCHC11

ZCCHC11 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 20119-1-AP

Synonymes

TUTase 4, TUT4, Terminal uridylyltransferase 4, PAPD3, KIAA0191

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Galerie de données de validation

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  WB analysis of HeLa using 20119-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20119-1-AP (ZCCHC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 20119-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 20119-1-AP (ZCCHC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20119-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20119-1-AP (ZCCHC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 20119-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20119-1-AP (ZCCHC11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 20119-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20119-1-AP (ZCCHC11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 20119-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20119-1-AP (ZCCHC11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 20119-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20119-1-AP (ZCCHC11 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 20119-1-AP

  Immunofluorescent analysis of Hela cells, using ZCCHC11 antibody 20119-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral humain
• Résultats positifs en IHC: tissu cérébral humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

20119-1-AP cible ZCCHC11 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZCCHC11 Protéine recombinante Ag13661
• Nom complet: zinc finger, CCHC domain containing 11
• Masse moléculaire calculée: 185 kDa
• Poids moléculaire observé: 185 kDa
• Numéro d’acquisition GenBank: BC048301
• Symbole du gène: ZCCHC11
• Identification du gène (NCBI): 23318
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ZCCHC11, also named as KIAA0191 and TUT4, is an uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. ZCCHC11 can't bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.

Publications

Species	Application	Title
human	WB,IF	Neoplasia; Identification of novel targets of miR-622 in hepatocellular carcinoma reveals common regulation of cooperating genes and outlines the oncogenic role of zinc finger CCHC-type containing 11.; Authors - Anne Gaza

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